How does a protein in buffer stick to a plastic plate
In order to stick antibodies on to a plastic plate, we put the antibodies
in a buffer and let it sit overnight at 4 C. l was wondering what the
mechanism of this adhesion is, does it reach equillibrium? Is it east to
get off if you wanted to remove it without necessarily destroying it?
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If you are thinking about ELISA plates (96-well plates), then
they are coated with a special chemical which become ''active'' at basic
pH. If I am right, then you must use either Carbonate or Borate buffer, pH
9, to coat your proteins onto the plates. These buffers (and their pH)
activate the compound which covers the plate and make it ''stick'' to the
hydrophobic groups of your proteins. It is not specific and it may change
the structure of some of the coated protein.
Bonding is through hidrophobic groups present in the protein
molecule.
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